Apr_05_237 - Name Student Number THE UNIVERSITY OF MANITOBA...

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Name: ________________________________ Student Number: __________________ _______________________________________________________________________________________ THE UNIVERSITY OF MANITOBA April 19, 2005, 9:00 AM - 12:00 PM Page 1 (of 4) Paper # 312 / 313 Biochemistry II Laboratory Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ 1. Answer ALL questions in the space provided . 2. The back side of each page may be used for your answer or for preliminary work. _______________________________________________________________________________________ Marks 2 1. State which chemicals or products were used in the lab this term for each of the following purposes. N.B. Full names and correct spelling required. A) To visualize lactate dehydrogenase by changing from colourless to purple in the staining sequence: Ans_____________________________________________________________________ B) To form the polar phase for adsorption chromatography: Ans_____________________________________________________________________ C) To act as the substrate for the assay of lysozyme: Ans_____________________________________________________________________ D) To act as the solvent to extract plant pigments: Ans_____________________________________________________________________ 3 2. Cellulose acetate electrophoresis is to be used to separate the following mixture of proteins: Protein A, pI 4.5 and MW 163,000; Protein B, pI 6.0 and MW 156,000; Protein C, pI 8.3 and MW 148,000. What would be the best pH to use to run the electrophoresis? Use a clearly labeled diagram to show the results you would expect to see and give reasons to justify the position of each protein.
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THE UNIVERSITY OF MANITOBA April 19, 2005, 9:00 AM - 12:00 PM Page 2 (of 4) Paper # 312 / 313 Biochemistry II Laboratory Section Final Examination Microbiology/Chemistry 60.237/2.237 Examiner: Dr. A. Scoot _______________________________________________________________________________________ Marks
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This note was uploaded on 08/07/2010 for the course CHEM 2378 taught by Professor Mark during the Spring '10 term at Manitoba.

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Apr_05_237 - Name Student Number THE UNIVERSITY OF MANITOBA...

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