Bacterial Isolation

Bacterial Isolation - IPHY 3060 Lab Bacterial Plasmid...

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IPHY 3060 Lab Bacterial Plasmid Isolation Lab
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The Purpose of Today’s Lab: To learn how plasmid DNA can be grown up and purified from bacteria for use in cell physiology experiments
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Bacteria—the Work Horse of Molecular Biology Bacteria grow extremely rapidly Bacteria can readily take up and replicate plasmid DNA Plasmid DNA can be isolated from bacteria very easily
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The Plasmid A circular piece of DNA that can be taken up by bacteria Plasmids typically contain 3 things: 1) An origin of replication so they can be replicated 2) The coding region for an antibiotic resistance gene 3) A region where an exogenous piece of DNA can be inserted
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Bacteria are grown in a liquid medium called Luria-Bertani (LB) broth that contains all the nutrients, ions, etc., that bacteria need to survive In addition, to prevent the growth of bacteria that don’t have our plasmid in it, we add the appropriate antibiotic to this medium. Bacteria need to be grown at 37ºC for optimal
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This note was uploaded on 08/11/2010 for the course IPHY 3060 taught by Professor Allen,davi during the Fall '09 term at Colorado.

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Bacterial Isolation - IPHY 3060 Lab Bacterial Plasmid...

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