Unformatted text preview: distance to this first stop will change as volume changes. The second stop is used for complete discharge of the solution from the plastic tip. 4.) Inaccurate volume setting, not allowing the pipet tip to fill to final volume and thus producing bubbles, and inserting the pipet too deep into the solution as opposed to just below the surface. Microscopes 5.) The 10X objective gives the widest field of view. 6.) The 1000X objective requires the most light. 7.) Lens paper should always be used in order to prevent scratching of the lens....
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- Fall '09
- James Petersen, T.A. James Petersen, Caroline O’Connor Lab