Unformatted text preview: In the plasmid DNA isolation procedure, the cell wall was broken down by lysozyme and burst open by heat, whereas in the mitochondrial DNA procedure, the cell wall was burst open by using heat alone • In the plasmid DNA isolation procedure, plasmid DNA was grown in large amounts through transformation of a bacteria that will divide and the DNA will be copied with every division. In the mitochondrial DNA isolation procedure, a polymerase chain reaction was used to amplify the mitochondrial control region and thus produce large amounts of mitochondrial DNA The procedures were similar in the following ways: • A centrifuge or microfuge were used in both for DNA isolation • Heat was used in both to aid in the rupturing of the cell wall • Mitochondria contain a circular piece of DNA similar to a plasmid containing some but not all of the genes for producing mitochondrial RNAs and, as a result, proteins • Mitochondrial DNA uses similar codon usage as bacteria...
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- Fall '09
- DNA, Mitochondrial DNA, DNA isolation procedure, Mitochondrial DNA PCR Lab