LAB6_OCON - Caroline OConnor Lab 6 T.A. James Peterman...

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Caroline O’Connor Lab 6 T.A. James Peterman TITLE: Muscle Immunohistochemistry Lab 1.) Only one of the slides, the G slide stained with type II myosin antibody, showed stained muscle fibers. This slide showed more darkly stained muscle fibers than light muscle fibers. Technically, all of the slides should have contained some stained muscle fibers for both the type I and type ii antibodies. The fact that the staining was not effective in all of our slides could be due to the fact that the primary antibody was not specific enough to bind to the protein. This, therefore, would not have allowed the secondary antibody to bind to the primary anitbody, and consequently would not have allowed the HRP to for the visible DAB precipitate. The lack of visible staining could have also been due to overwashing of the slide with the primary antibody and consequently resulting in too little primary antibody left on the slide to recruit the binding of the secondary antibody and so forth.
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This note was uploaded on 08/11/2010 for the course IPHY 3060 taught by Professor Allen,davi during the Fall '09 term at Colorado.

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LAB6_OCON - Caroline OConnor Lab 6 T.A. James Peterman...

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