Lab10_OCON - Caroline OConnor Cell Physiology Lab 10 T.A...

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Caroline O’Connor Cell Physiology Lab 10 T.A. James Peterson Title: Cell Culture Differentiation Lab: Differentiation of muscle cells in culture 1.) All of the cells are not differentiated into myotubes. The cells in panel A are still amorphous. Therefore, they can be classified as undifferentiated myblasts. The cells in panel B, however, are cylindrical in shape, and have differentiated into mytubes. The reason that the cells in panel A have not yet differentiated into myotubes is because differentiation happens gradually over time as a result of different stimuli such as contact inhibition. In the slides represented in the power point, we are only observing a snapshot in time, and these two snapshots were most likely taken at different stages, therefore showing different degrees of differentiation. 2.) The cells in panel A are multi-nucleated and appear to have one continuous cytoplasm. Therefore, these cells represent differentiated myotubes. The cells in panel B only have a single nucleus surrounded by a distinct cytoplasm. These cells have not yet differentiated into myotubes, and can still be classified as undifferentiated myoblasts. 3.) The cells in panel A display a small amount of red staining that represents contractile
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This note was uploaded on 08/11/2010 for the course IPHY 3060 taught by Professor Allen,davi during the Fall '09 term at Colorado.

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Lab10_OCON - Caroline OConnor Cell Physiology Lab 10 T.A...

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