6A_Lecture_7_with_ink

6A_Lecture_7_with_ink - Lecture 7 Liquid Chromatography Gas...

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Lecture 7
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Liquid Chromatography Gas Chromatography Mass Spectrometry (MS) Experiment 7
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Stationary phases (adsorbents) SiO 2 X H 2 O (Silica Gel) most common Water plays key role in separation Al 2 O 3 Used for low to med polarity separations Can react with compounds (Lewis Acid)
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Stationary Phases (continued) Reverse Phase chromatography Used for very polar compounds Most polar compounds elute first Very expensive
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Solvent Selection Perform TLC experiments until: 1. There is good separation between spots 2. The desired product spot has an R f of about 0.3 Solvents must be purified Small amounts of impurities can cause changes in polarity of the solution Flash Chromatography Air forces the liquid through the column to make it come out faster
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GC is actually Gas-Liquid Chromatography
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Stationary phase Made up of a liquid polymer coated in column Inside of the column is open A carrier gas (helium) carries the gas through
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6A_Lecture_7_with_ink - Lecture 7 Liquid Chromatography Gas...

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