CHEM 6A Lecture

CHEM 6A Lecture - CHEM 6A Lecture August 9 2010 Liquid...

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CHEM 6A Lecture 22:09 August 9, 2010 Liquid chromatography Stationary phases (adsorbents) o Silica gel is most common o Water plays key role in seperation o Al2O3, Alumina, Cellulose Cellulose very polar o Used for low to med polarity separatiosn o Can react with compounds Reverse phase o Chromatography o Used for very polar compounds o Most polar compounds elute first o Very expensive Solvent selection o Perform tlc until: Good separation between spots Desired product spot has Rf of about .3 Solvent must be purified Sand: don’t have Gas Chromatography Stationary phase Carrier gas helium carries gas through column o Partitioning
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o Compounds separate based on affinity for stationary phase o Temperature o Flow rate Types of columns o Capillary column open in center o Packed columns closed/packed o Diatomaceous polar Types of stationary phases How to read: o First peak is air 1. Retentive 2. Area o use quantitative method o order of boiling point for elution if using a non-polar type of environment
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This note was uploaded on 08/14/2010 for the course BIO 2010 taught by Professor Le during the Summer '10 term at UCSB.

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CHEM 6A Lecture - CHEM 6A Lecture August 9 2010 Liquid...

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