BLH24-spring10

BLH24-spring10 - B. differences in their size, large DNA...

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BCH 100, Fall 2010 BLH #24 4 points LECTURE #26 due Wed., June 2, 2010 at 2 pm 1. (1 pt) Which of the following DNA sequences in its double-stranded form is likely to be a target sequence for cleavage by a restriction endonuclease? A. 5’ …ACGACG… 3’ B. 5’ …ACGGCA… 3’ C. 5’ …ACGCGT… 3’ D. all of the above E. none of the above 2. (1 pt) Commonly used vectors for DNA cloning include A. restriction enzymes. B. DNA polymerase from Thermus aquaticus. C. plasmids. D. phages (bacterial viruses). E. both A and B. F. both C and D. G. all of the above. H. none of the above. 3. (1 pt) “Sequencing gels” (electrophoresis of mixtures of DNA fragments on polyacrylamide or agarose gels) separate DNA molecules by A. differences in their size, small DNA fragments moving further in the gel than larger fragments.
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Unformatted text preview: B. differences in their size, large DNA fragments moving further in the gel than smaller fragments. C. differences in their charge, larger DNA fragments carrying more negative charges than smaller fragments and thus moving faster. D. differences in their charge, smaller DNA fragments carrying fewer negative charges than larger fragments and thus moving faster. E. none of the above 4. (1 pt) If you run a dideoxy DNA sequencing gel, the sequence of bases you read from the gel is the sequence of A. the template strand, 3 to 5, from bottom to top of gel. B. the template strand, 5 to 3, from bottom to top of gel. C. the newly synthesized strand, 3 to 5, from bottom to top of gel. D. the newly synthesized strand, 5 to 3, from bottom to top of gel. E. none of the above...
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