Ion channels Part1

Ion channels Part1 - Michael Goryll Department of...

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Michael Goryll Department of Electrical Engineering August 17, 2010 Lecture Notes: Experimental studies of ion permeation through trans-membrane channels making electrical contacts to cells the “patch-clamp” technique ion channel reconstitution into artificial membranes
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Introduction Introduction certain (non-heart related) drugs cause Q-T prolongation, which causes life threatening arrythmia Finlayson et al. European Journal of Pharmacology 500 (2004) 129– 142
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Introduction Introduction long Q-T syndrome is caused by a mutated potassium ion channel that is switched open by the drug, increasing I Kr To date, electrophysiology remains the ‘gold standard’ method with which to characterize ion channel properties” Finlayson et al. European Journal of Pharmacology 500 (2004) 129– 142 Witchel et al. Journal of Pharmacological and Toxicological Methods 48 (2002) 65– 80 the FDA introduced mandatory testing for every new drug against long Q-T syndrome effects !
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August 17, 2010 Images from Purves et al., Life: The Science of Biology, 4th Edition, by Sinauer Associates and WH Freeman amphiphilic molecules = polar headgroup + hydrocarbon tail (hydrophilic) (hydrophobic) hydrophobic tails join to exclude water molecules ~ 4 nm Cell membrane – Lipid bilayer Cell membrane – Lipid bilayer
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August 17, 2010 Image from R. Waser, Nanoelectronics and Information Technology, Chapter 6, 1st Edition, by Wiley-VCH ions are surrounded by a cloud of water molecules ions do not permeate the lipid bilayer ion channels allow the control of an ionic current through a highly insulating lipid bilayer membrane (10 15 cm) ion channel activity is related to ion permeation currents Cell membrane – Ion Channels Cell membrane – Ion Channels
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August 17, 2010 ion channels can be studied by measuring a transmembrane current or a transmembrane voltage, keeping the other quantity constant both sides
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Ion channels Part1 - Michael Goryll Department of...

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