Ion channels Part2

Ion channels Part2 - Michael Goryll Department of...

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Michael Goryll Department of Electrical Engineering Arizona State University Ion Channel Biosensors on Silicon
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outer membrane protein of E.coli structure investigated by XRD easy to isolate from bacterium inserts as trimer into membrane 7 x 10 6 copies per cell side view top view S. W. Cowan, T. Schirmer, G. Rummel, M. Steiert, R. Ghosh, R. A. Pauptit, J. N. Jansonius, J. P. Rosenbusch, 358(6389), 727 (1992). Ion Channels - OmpF Ion Channels - OmpF
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charge distribution profile : molecular dynamic simulations voltage gated K + channel “negative differential resistance” Image from R. Waser, Nanoelectronics and Information Technology, Chapter 6, 1st Edition, by Wiley-VCH H. Schindler and J. P. Rosenbusch, Proc. Natl. Acad. Sci. USA 75(8), 3751 (1978). Image courtesy of S. Aboud and M. Saraniti, IIT Image from H. R. Petty, Molecular Biology of Membranes, Chapter 2, by Plenum Press Ion Channels - OmpF Ion Channels - OmpF
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Staphylococcus aureus protein various gating mechanisms (voltage / ligand gated, DNA translocation) easy to genetically engineer heptameric channel protein side view top view L. Song, M. R. Hobaugh, C. Shustak, S. Cheley, H. Bayley, J. E. Gouaux, Science, 274(5294), 1859 (1996). Ion Channels – Ion Channels – α α -Haemolysine -Haemolysine
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Ion Channels as Sensors Ion Channels as Sensors Orit Braha et al., Nature Biotechnology 18 , (2000) R. Syeda et al. , J. Am. Chem. Soc., 130 (46), 15543-15548,(2008) Guan et al., ChemBioChem 6 , 1875–1881, (2005) Metal ion detection S. Howorka and H. Bayley, Biophysical Journal 83 , 3202–3210 (2002) RNA/DNA sequence readout Explosives detection Drug screening
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Ion Channel Array Ion Channel Array frequency of binding event reveals concentration of analyte duration and amplitude reveals identity of analyte array platform : engineered pores with different sensitivity sensing in parallel, ionic current signal pattern processed by DSP s H. Bayley and P. Cremer, Nature 413, 226-230 (2001)
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http://warnerinstruments.com a polystyrene cup with a mechanically machined aperture (150 µm diameter) is used to span bilayers across chlorided silver wires are used as electrical contacts Aperture Polystyrene Cup Recess Glass Window Upper image from Williams, Alan, Ion Channels A Practical Approach Wells Current Technology Current Technology
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Silicon Platform Silicon Platform possibility to use well established microfabrication technology (photolithography, dry etching, evaporation) reproducibility scalability down to µm-sized apertures repeatability on a single substrate Lipid Bilayer with Ion Channels Pathway towards a fully
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Ion channels Part2 - Michael Goryll Department of...

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