Ion channels Part3

Ion channels Part3 - Michael Goryll Department of...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Michael Goryll Department of Electrical Engineering Lecture Notes 3 : Micro- and nanofabrication for planar patch-clamp and ion channel reconstitution polymer membranes (molding, ion-track etching) glass chips silicon (-dioxide, -nitride) chips for suspended planar bilayers
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Lipid Bilayer Thinning Lipid Bilayer Thinning Niles et al. Biophysical Journal 53, 327-335(1988). Bath Bilayer
Background image of page 2
Patch-clamp Integration Patch-clamp Integration patch-clamp is the “gold standard” for electrophysiological measure- ments the method is fairly complex and requires experienced scientists mandatory drug screening calls for high-throughput tools fully automated tools without manual interaction are preferred faster measurements must not sacrifice accuracy low cost (smaller than $1 per measurement) Sigworth and Klemic, IEEE TRANSACTIONS ON NANOBIOSCIENCE, VOL. 4, NO. 1, MARCH 2005 How can we locate cells ? How can we mass-produce patch pipettes ? How small can we make the pipettes ?
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
AFM Patch-Clamp AFM Patch-Clamp Gorelik et al. Biophysical Journal Volume 83 December 2002 3296 3303 patch pipette is used as an AFM tip to locate cells still only one measurement per pipette feasible
Background image of page 4
State-of-the-art HTS State-of-the-art HTS commercially available is the IonWorks TM HT platform from Molecular Devices it uses an assembled plastic chip to replace the patch pipette cells are loaded by a robot onto the chip and hold by vacuum 348 measurement sites per chip few (up to 16) parallel recordings
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 24

Ion channels Part3 - Michael Goryll Department of...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online