Unformatted text preview: 33 '__ in region indicates this is a prokaryotic gene. 50. The start of translation codon is idenfﬁ'ed as region
a. l Luna's.) sign 9' 51. The stop codon is identiﬁed in region 52. Transcription of this gene would begin in region
e. S 53. If you had a linear DNA molecule that was 100,000 base pairs long, and you wanted to cut this
into just two pieces, your best chance of doing this would be with a restriction enzyme that has a
base pair recognition sequence. (:4, If you had many copies of a clone corresponding to a particular DNA fragment, and you 'yanted to obtain overlapping fragments of the insert to subclone, this would best be accomplished
through : a. a complete digest with a restriction enzyme producing 3' overhangs b. a complete digest with a restriction enzyme producing 5’ overhangs ._:__§ a complete digest with a restriction enzyme producing blunt ends it} a partial restriction enzyme digest V". impossible to generate overlapping ﬁagments in a restriction enzyme digest Page 12 of 22 ...
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- Spring '10