Bio208SampleMidterm

Bio208SampleMidterm - Biology 208 Midterm Exam Fall 2007...

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1 Biology 208 Midterm Exam Fall 2007 Answers 1. Define the following: (2 marks each – do not write more than about 2 sentences.) a. Bioinformatics b. Probe c. Tm d. Reverse genetics 2. Explain the following statements: (2 marks each) a. It is essential to wear gloves when handling gels stained with ethidium bromide.(2 marks for the concept) b. List 4 types of information that biochemical analysis might reveal about a purified enzyme. (2) 3. Explain how to do the following: (use the marks to estimate the amount of detail needed) 1. How could you determine if your plasmid DNA has been linearized following incubation with a restriction enzyme? (3) 2. List the best method to do the following: (3) Inactivation of protein/enzymes (1 method) Removal of proteins (2 methods)
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2 3. Explain the basis for the following: (ie how do they work?) (marks in brackets) a. A schematic standard curve of distance migrated versus fragment size is shown below. Explain the migration patterns indicated by the thick lines . (2 marks for the concept) b. Microorganisms protect their genomes from pathogen attack by host restriction-modification. What
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This note was uploaded on 08/18/2010 for the course BIOL BIOL208 taught by Professor Chuong during the Spring '10 term at Waterloo.

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Bio208SampleMidterm - Biology 208 Midterm Exam Fall 2007...

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