BIBC 102 Exam 2B KEY

BIBC 102 Exam 2B KEY - Metabolic Biochemistry Summer...

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Metabolic Biochemistry KEY Summer Session 2010 Exam 2 (480 pts.) Version B Section 1 DO THIS PART OF THE EXAM FIRST 1) (12 pts.) The complete oxidation of glucose yields (fill in the blanks and balance the equation): C 6 H 12 O 6 + 6O 2 6 CO 2 + 6 H 2 O 2) (6 pts.) For both lactate and ethanol fermentation (the conversion of pyruvate to lactate or ethanol under anaerobic conditions), what gets recycled that allows glycolysis to continue functioning? NADH to NAD + (half credit for only one; zero credit for backwards) 3) (6 pts. each for 36 pts.) Indicate whether each statement about metabolism under anaerobic conditions is true (T) or false (F). When no oxygen is present in the cell: T Electrons would stop flowing through the electron transport chain. T In mammalian cells, pyruvate would be continually converted to lactate as long as a constant source of glucose was present. F All stages of glucose oxidation shut down when no oxygen is present, and no ATP can be produced from glucose. F In muscle cells, pyruvate would be converted to ethanol so that glycolysis could keep functioning. T ATP synthesis by substrate-level phosphorylation could continue as long as a constant source of glucose was present. F ATP synthesis by oxidative phosphorylation could continue as long as a constant source of glucose was present. 4) (6 pts. each) Give the name of the cellular compartment or organelle where each of the following processes occur? Be as specific as possible. a. Beta-oxidation of fatty acids Matrix of mitochondria (one or the other is okay) b. Glycolysis Cytoplasm (or cytosol) c. The citric acid cycle Matrix of the mitochondria
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5) (6 pts.) Why do all (or almost all) naturally occurring fatty acids contain an even number of carbon atoms? Answer by telling how this relates to how fatty acids are synthesized and oxidized. Synthesized from 2-carbon acetyl groups from acetyl-CoA 6) (50 pts.) Glucose is oxidized by glycolysis and cellular respiration to yield ATP and reduced electron carriers that power ATP synthesis through the electron transport chain. Fill in the blanks below to give the numbers of these products produced at each stage of glucose oxidation. (5 pts. each) Give the numbers that are produce per molecule of glucose that enters glycolysis. GLYCOLYSIS: Glycolysis produces 2 molecules of ATP and 2 molecules of NADH per glucose. How many ATP are produced from all the NADH produced in glycolysis (per glucose)? Give the maximum number only. 5 TOTAL ATP PER GLUCOSE FOR GLYCOLYSIS: __7 __ PYRUVATE DEHYDROGENASE COMPLEX: At the PDH complex, 2 molecules of NADH are produced per glucose. How many ATP are produced from all the NADH produced by the PDH complex (per glucose)? 5 TOTAL ATP PER GLUCOSE FOR PDH COMPLEX: __5 ____ CITRIC ACID CYCLE: The citric acid cycle produces 6 molecules of NADH per glucose. How many ATP are produced from all the NADH produced by the citric acid cycle?
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BIBC 102 Exam 2B KEY - Metabolic Biochemistry Summer...

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