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2009 Form B - AP Biology 2009 Scoring Guidelines Form B The...

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AP ® Biology 2009 Scoring Guidelines Form B The College Board The College Board is a not-for-profit membership association whose mission is to connect students to college success and opportunity. Founded in 1900, the association is composed of more than 5,600 schools, colleges, universities and other educational organizations. Each year, the College Board serves seven million students and their parents, 23,000 high schools and 3,800 colleges through major programs and services in college readiness, college admissions, guidance, assessment, financial aid, enrollment, and teaching and learning. Among its best-known programs are the SAT ® , the PSAT/NMSQT ® and the Advanced Placement Program ® (AP ® ). The College Board is committed to the principles of excellence and equity, and that commitment is embodied in all of its programs, services, activities and concerns. © 2009 The College Board. College Board, Advanced Placement Program, AP, AP Central, SAT, and the acorn logo are registered trademarks of the College Board. PSAT/NMSQT is a registered trademark of the College Board and National Merit Scholarship Corporation. Permission to use copyrighted College Board materials may be requested online at: www.collegeboard.com/inquiry/cbpermit.html. Visit the College Board on the Web: www.collegeboard.com. AP Central ® is the official online home for AP teachers: apcentral.collegeboard.com.
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AP ® BIOLOGY 2009 SCORING GUIDELINES (Form B) © 2009 The College Board. All rights reserved. Visit the College Board on the Web: www.collegeboard.com. Question 1 Describe how a plasmid can be genetically modified to include a piece of foreign DNA that alters the phenotype of bacterial cells transformed with the modified plasmid. Describe a procedure to determine which bacterial cells have been successfully transformed. Describe plasmid modification (8 points maximum) : Topic Description (1 point each) Plasmid vector Describes plasmid as small circular DNA Cut (cleave) DNAs Use of restriction endonucleases (RE) Plasmid and inserted DNA must have same RE cut ends or be cut by same RE Sticky ends Ends of DNA should be sticky, wanting to bond with matching ends Generate ends for attachment using endonucleases Ligase For joining of sticky ends Orientation Correct orientation of insertion to ensure expression Gene of interest DNA cut should be a complete sequence of gene Attach piece with a promoter or insert next to promoter Reporter gene Gene used to identify insertion of desired DNA Insert DNA with a gene that produces a new phenotype Selective marker Inserted to help identify the DNA insertion (e.g., antibiotic resistance) AUG in place Ensure proper start codon Uptake of plasmid Calcium chloride and heat shock, electroporation to make competent Alternative procedures
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