Colley - Lec 10 Protein Trafficking I - Part A and B - 2010

Colley - Lec 10 Protein Trafficking I - Part A and B - 2010...

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Unformatted text preview: Lecture 10 Lecture 10 Part A Part A Protein Trafficking I : Entry into the secretory pathway Protein folding and modification Diseases related to aberrant glycosylation Karen Colley 2020 MBRB karenc@uic.edu Entry into the Secretory Pathway: Entry into the Secretory Pathway: Translocation Across the ER Membrane Translocation Across the ER Membrane Mitochondria Mitochondria Ribosomes Ribosomes Cytoplasm Cytoplasm Endoplasmic Reticulum Endoplasmic Reticulum Peroxisomes Peroxisomes Nucleus Nucleus Golgi Golgi Lysosomes Lysosomes Secretory Secretory Granules Granules Plasma Membrane Plasma Membrane Signal Peptides Direct Proteins into Signal Peptides Direct Proteins into the Secretory Pathway via the ER the Secretory Pathway via the ER General Characteristics of Signal Peptides General Characteristics of Signal Peptides Location Location : Found at the amino terminus of the protein and are first to : Found at the amino terminus of the protein and are first to emerge from the ribosome during translation. emerge from the ribosome during translation. Length Length : 16-22 amino acids 16-22 amino acids Character Character : Polar/basic amino terminus : Polar/basic amino terminus Central hydrophobic core Central hydrophobic core (6-8 amino acids) (6-8 amino acids) Relatively hydrophilic carboxy-terminus with cleavage site Relatively hydrophilic carboxy-terminus with cleavage site Cleavage site Cleavage site : : Small uncharged amino acids Small uncharged amino acids (i.e. Ala, Cys, Gly, Ser) (i.e. Ala, Cys, Gly, Ser) at positions -1 and -3 from cleavage site (no Pro, charged, at positions -1 and -3 from cleavage site (no Pro, charged, large, aromatic residues in region of -3 to -1). large, aromatic residues in region of -3 to -1). Preproalbumin Met-Lys-Trp-Val-Thr- Preproalbumin Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser--Ser-Gly-Ser-Ala Ala-Phe--Phe-Ser Ser-Arg-Arg Prelysozyme Met-Arg-Ser- Prelysozyme Met-Arg-Ser-Leu-Leu-Ile-Leu-Val-Leu-Cys-Phe-Leu- Leu-Leu-Ile-Leu-Val-Leu-Cys-Phe-Leu- Pro-Leu-Ala- Pro-Leu-Ala-Ala Ala-Leu--Leu-Gly Gly-Lys-Lys VSV G precursor Met-Lys-Cys- VSV G precursor Met-Lys-Cys-Leu-Leu-Tyr-Leu-Ala-Phe-Leu-Phe-Ile Leu-Leu-Tyr-Leu-Ala-Phe-Leu-Phe-Ile-His--His-Val Val-Asn--Asn-Cys Cys-Lys-Lys Most Secretory Pathway Proteins are Co-translationally Translocated Across the ER Membrane Targeting and Translocation of Nascent Proteins Targeting and Translocation of Nascent Proteins To and Across the ER Membrane: To and Across the ER Membrane: Components of the Process Components of the Process Signal Recognition Signal Recognition Particle (SRP) Particle (SRP) Signal peptide Signal peptide SRP Receptor SRP Receptor Translocation Channel Translocation Channel (Sec 61 +TRAM) (Sec 61 +TRAM) Signal peptidase complex Signal peptidase complex SRP directs the nascent secretory protein and ribosome to the ER membrane where it interacts with the SRP receptor After docking with the receptor, SRP transfers the nascent protein to the...
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This note was uploaded on 08/21/2010 for the course BMS 652 taught by Professor Dr.yee-kinho during the Fall '10 term at University of Illinois at Urbana–Champaign.

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Colley - Lec 10 Protein Trafficking I - Part A and B - 2010...

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