Colley - Lec 11- Protein Trafficking II - 2010

Colley - Lec 11- Protein Trafficking II - 2010 - Lecture 11...

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Unformatted text preview: Lecture 11 Protein Trafficking II Mitochondrial Targeting and Localization Nuclear Import and Export Karen Colley 2020 MBRB [email protected] General Biosynthetic Protein Traffic: Post-translational Targeting from the Cytosol Ribosomes Ribosomes Cytosol Cytosol Endoplasmic Reticulum Endoplasmic Reticulum Mitochondria Mitochondria Peroxisomes Peroxisomes Nucleus Nucleus Golgi Golgi Lysosomes Lysosomes Secretory Secretory Granules Granules Plasma Membrane Plasma Membrane Protein Import into Protein Import into the Mitochondria the Mitochondria Most Mitochrondrial Proteins are Synthesized in the Cytosol as Precursors Only about 10 proteins are encoded by mitochondrial DNA. The rest (~98%) are encoded by nuclear DNA. These proteins are transported into the mitochondria post-translationally. I nter membr ane Membr ane Space Matr ix Outer Membr ane I nner Membr ane Found at the amino terminus ~15-30 amino acids long Rich in basic (+) and hydroxylated amino acids Fold into an amphipathic helix (one face hydrophobic-one face +charged). Proteins destined for a location other than the matrix will also frequently have second targeting signal. Mitochondrial Protein Sorting Signals (Uptake-Targeting Signals) General Scheme of Mitochondrial Import Receptor TOM channel TIM channel Cytosolic chaperone mhsp70 Mitochondrial processing protease Preprotein Folded protein hsp60 1. A preprotein is maintained in a translocation competent state by cytosolic chaperones (hsp 70). 2. The preprotein is directed to a membrane receptor on the outer membrane....
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  • Fall '10
  • Dr.Yee-KinHo
  • Localization Nuclear Import and Export, ~15-30 amino acids long Rich in basic, Mitochondrial Membrane Translocation, Mitochondrial Membrane Translocation Systems

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Colley - Lec 11- Protein Trafficking II - 2010 - Lecture 11...

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