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Review Guide - Lecture 3 - Body Fluid - 2010

Review Guide - Lecture 3 - Body Fluid - 2010 - Review Guide...

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Review Guide and Self Assessment Quiz Body Fluids Acid-base chemistry and buffer Water and electrolyte balance Harper’s – Chapter 2 – Water and pH Clinical Biochemistry – Core Biochemistry – Fluid and electrolyte balance Acid-base concepts Metabolic acid-base disorders
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Concept, Knowledge and Objectives Body fluid – Water content, pH, electroyltes and osmolality. Extracellular and intracellular fluid. Definition of pH, pKa and buffer. Application of Henderson- Hasselbalch equation Calculation of pH and concentration of buffer capacity. pI and its implications in protein structure. Its relationship to pH and charges on the molecule. Acids production in the body – Normal plasma pH, stomach fluid. The role of carbonic anhydrase in CO 2 -bicarbonate equilibrium. Its role in tissues, lung and red cells. Buffering molecules in our body. The role of lung, kidney and red cells in pH homeostasis. The importance of maintaining oncotic pressure. Electrolyte concentration in plasma – 150 mM salt. The concept of “Anion Gap” Water balance in edema and dehydration – Physical symptom.
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The following diagram is the titration curve for amino acid glycine.
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