MOLECULAR BIOLOGY v2

MOLECULAR BIOLOGY v2 - MOLECULAR BIOLOGY A.P LAB 6...

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MOLECULAR BIOLOGY A.P. LAB # 6
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INTRODUCTION/BACKGROUND INFORMATION: In this lab we learned how plasmids containing specific fragments of foreign DNA will be used to transform E. coli cells, and also how restriction enzyme digests of phage lambda DNA are used to demonstrate techniques for separating and identifying DNA fragments using gel electrophoresis. Plasmids are extrachromosomal and some can replicate only when the bacterial chromosome replicates and usually exist only as single copies within the bacterial cell. Before doing this lab we learned how to do gel electrophoresis and separate DNA molecules present in a mixture based on size and also on polarity. We also learned how restriction enzymes function and are extremely important to genetic engineering experiments. MATERIALS: See lab packet six for the materials for each lab exercise. PROCEDURE: See lab packet six for the procedure for each lab exercise.
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