CELL RESPIRATION v2

CELL RESPIRATION v2 - CELL RESPIRATION A.P. LAB # 5...

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CELL RESPIRATION A.P. LAB # 5
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INTRODUCTION/BACKGROUND INFORMATION: In this lab our objective was to measure the rate of aerobic respiration over a time period of twenty minutes for germinating and nongerminating pea seeds. In this experiment we tested and measured the relative volume of oxygen consumption by the germinating and nongerminating peas in two different temperatures, a 25 degree Celsius atmosphere, and a 10 degree Celsius atmosphere. Aerobic cellular respiration is the release of energy from organic compounds by metabolic chemical oxidation, and it involves many enzyme-catalyzed reactions. There are three ways in which cellular respiration can be measured, but in this lab we focused on measuring oxygen consumption over a certain period of time using a respirometer. The respirometer was attached to our vials and the carbon dioxide produced during the cellular respiration was removed by potassium hydroxide (KOH) which we put in the bottom of our vials, however, not touching the peas. To make our data collection more accurate, we had a control vial filled with glass beads and this helped in keeping our data exact. MATERIALS: See lab packet 5 for the materials. PROCEDURE: See lab packet 5 for the procedure.
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DATA: Raw data from this lab is in the lab packet, and the graph and table are attached on page 8. ANALYSIS: 1. In this activity we investigated both the effect of germination versus nongermination and warm temperature versus cold temperature on respiration rate. Two hypotheses being tested in this activity are; the germinating peas will have a much higher rate of cellular respiration than the nongerminating (dry) peas, and the respiration rate will be higher in a warmer temperature versus a colder temperature. 2. This activity used many controls. They conditions that had to remain constant were temperature, volume, amount of cotton, amount of KOH, size of the respirometer, and the number of peas in each respirometer. Temperature must remain constant within the experiment because it will change the pressure in the respirometer. Total volume must remain constant because we wouldn’t be able to compare the three experiments if the volumes were not identical. Amount of cotton in each respirometer must remain constant because if not the volumes for each respirometer would be different. There needed to be the same amount of KOH in each respirometer so that the same amount of carbon dioxide is being removed from the vials. The size of the respirometers must be constant so that the volume is the same and temperature would be
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dispersed evenly. The number of peas in each respirometer must be
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CELL RESPIRATION v2 - CELL RESPIRATION A.P. LAB # 5...

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