ENZYME CATALYSIS v2 - ENZYME CATALYSIS A.P. LAB # 2 NAME...

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ENZYME CATALYSIS A.P. LAB # 2 NAME CLASS DATE
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INTRODUCTION/BACKGROUND INFORMATION: In this lab our objective was to measure the rate of Hydrogen Peroxide consumption over time, and observe the chemical reaction of Hydrogen Peroxide and the enzyme Catalase with the products oxygen gas and water. This lab included the procedure of quantitative titration, and this helped us to find the rate of the enzyme-catalyzed reaction. We used a number of chemicals to complete this lab including Catalase, Hydrogen Peroxide, Sulfuric Acid, and Potassium Permanganate. By catalyzing the chemical reaction, the enzyme Catalase sped up the reaction rate between the enzyme and the substrate to yield the product. Enzymes are substrate specific, meaning they only react with certain substrates. Catalase is specific to Hydrogen Peroxide, the substrate in this reaction. Sulfuric Acid is known as an inhibitor because it decreases the reaction rate of Catalase and Hydrogen Peroxide. When added, the Sulfuric Acid lowers the pH, denatures the enzyme, and thereby stops the enzyme’s catalytic activity. Potassium Permanganate was used in this lab to indicate the amount of Hydrogen Peroxide remaining, and this is because Potassium Permanganate turns a pinkish brown color when in the presence of Hydrogen Peroxide. To begin the lab we found a baseline; how much Hydrogen Peroxide was in the starting beaker for the class. We also set aside
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This note was uploaded on 08/23/2010 for the course CS 1371 taught by Professor Stallworth during the Spring '08 term at Georgia Institute of Technology.

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ENZYME CATALYSIS v2 - ENZYME CATALYSIS A.P. LAB # 2 NAME...

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