PLANT PIGMENTS AND PHOTOSYNTHESIS v2

PLANT PIGMENTS AND PHOTOSYNTHESIS v2 - PLANT PIGMENTS AND...

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PLANT PIGMENTS AND PHOTOSYNTHESIS A.P. LAB # 4 NAME CLASS DATE
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INTRODUCTION/BACKGROUND INFORMATION: In this lab our purpose was to observe the separation of plant pigments using chromatography and to measure the rate of photosynthesis in isolated chloroplasts using the dye DPIP. We used paper chromatography because it is efficient at separation and identifying pigments from cell extracts. The solvent moves up the chromatography paper by capillary action, and as it moves up the paper, it carries along any substances dissolved in it. Chlorophyll A is the primary photosynthetic pigment located in the reaction center of photosystems. Other pigments, such as carotene, xanthophylls, and chlorophyll B are light capturing pigments that transfer the captured energy to chlorophyll A. In the second part of the lab we used our knowledge of the process of photosynthesis and the relationship between light wavelength or intensity and photosynthetic rate to explain why the rate of photosynthesis differs under various environmental conditions. We knew that the transfer of electrons during the light-dependent reactions of photosynthesis reduces DPIP, which changes it from blue to colorless. MATERIALS: See lab packet four for the materials for each lab exercise. PROCEDURE: See lab packet four for the procedure for each lab exercise.
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DATA: Raw data from this lab is in the lab packet, graphs included on page 6. ANALYSIS:
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This note was uploaded on 08/23/2010 for the course CS 1371 taught by Professor Stallworth during the Spring '08 term at Georgia Institute of Technology.

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PLANT PIGMENTS AND PHOTOSYNTHESIS v2 - PLANT PIGMENTS AND...

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