DIFFUSION AND OSMOSIS v2

DIFFUSION AND OSMOSIS v2 - DIFFUSION AND OSMOSIS A.P. LAB #...

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DIFFUSION AND OSMOSIS A.P. LAB # 1 NAME CLASS DATE
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INTRODUCTION/BACKGROUND INFORMATION: In this lab we did four different exercises to investigate the processes of diffusion and osmosis in the membrane system and to observe the effect of solute concentration on water potential as it relates to living plant tissues. In exercise 1B we used dialysis tubing to observe the process of osmosis in relation to solute concentration. We wanted to find out if there was a change in mass, and if so, what was the cause for this change. In exercise 1C we used potato cores placed in different molar concentrations of sucrose to determine the water potential of potato cells. To find the water potential of the potato cells we had to first find the percent change in mass of the potato cores at the different molar concentrations of sucrose. From there, we could determine the sucrose molarity in which the mass of the potato core does not change. With this value, and an equation to find the water potential of the potato cells, we could figure out what the water potential was numerically and what this meant in terms of the potato cells. In exercise 1D we actually calculated the water potential from the experimental data we gathered in part 1C. Water potential measures the tendency of water to leave on place in favor of another and is affected by pressure potential and solute potential. In exercise 1E we observed onion cell plasmolysis. Plasmolysis is the shrinking of the cytoplasm of a plant cell in response to diffusion of water out of the cell and into a hypertonic solution. In this lab exercise we examined the effects of highly concentrated solutions on diffusion and cellular contents.
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MATERIALS: See lab packet one for the materials for each lab exercise. PROCEDURE: See lab packet one for the procedure for each lab exercise. DATA: Raw data from this lab is in the lab packet. The graphs for each exercise are on page eight. ANALYSIS: 1A. 1. In this exercise, the substance entering the bag was water. Water entered the bag because it diffused from a higher concentration region, in the beaker, to a lower concentration region, in the dialysis bag. The glucose would diffuse out of the bag, from high concentration to low concentration, and it can fit through the pores of the dialysis bag because its molecules are tiny. When the beaker is tested for starch, it doesn’t appear because start cannot fit through the selectively
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This note was uploaded on 08/23/2010 for the course CS 1371 taught by Professor Stallworth during the Spring '08 term at Georgia Institute of Technology.

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DIFFUSION AND OSMOSIS v2 - DIFFUSION AND OSMOSIS A.P. LAB #...

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