LAB 4 v3 - LAB 4 NOTE: These labs are known for high...

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LAB 4 NOTE: These labs are known for high failure rates. Therefore, if I show an example using data, a graph, etc… yours may be totally different depending upon mine and yours success with the lab. So don’t freak if your data is completely different from mine. And therefore the analysis of results sometimes may need to be answered differently since you base your analysis off your data and not mine. DISCLAMIER: DO NOT CHEAT! Introduction: Photosynthesis has two main parts, which are the light dependent and the light –independent. In the light-dependent reactions pigments trap energy from light, and this energy is used to split water molecules (photolysis). The light- independent reactions or dark phase of photosynthesis involve the fixing of carbon dioxide. It makes glucose and fructose chains and also releases oxygen , which passes through the stomata of the plant. Organisms that carry out photosynthesis making their own organic molecules are called autotrophic. Some autotrophic organisms include plants, algae, and blue-green bacteria. Plants have many varieties of pigments, all of which absorb different colors of light. Chlorophyll a is the primary plant pigment and makes up about three-fourths of all the plant pigments. It absorbs red and blue light and is not found in photosynthetic bacteria. Chlorophyll b is another plant pigment. It absorbs blue-green and orange-red light. Carotenoids are a type of accessory pigment that absorb blue and blue- green light. These pigments are fat soluble and usually masked by chlorophyll a. Anthocyanin is another accessory pigment that absorbs bright red colors. There is also chlorophyll c and d that sometimes take the place of chlorophyll b. Chromatography is a process used to separate mixtures that can separate plant pigments. This lab uses paper chromatography where a piece of paper is used to wick solvent up to the pigments and separate them according to solubilities. The rate of migration on a chromatogram is the R f value. Hypothesis
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This note was uploaded on 08/23/2010 for the course CS 1371 taught by Professor Stallworth during the Spring '08 term at Georgia Institute of Technology.

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LAB 4 v3 - LAB 4 NOTE: These labs are known for high...

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