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LAB 4 v2 - LAB 4 NOTE These labs are known for high failure...

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LAB 4 NOTE: These labs are known for high failure rates. Therefore, if I show an example using data, a graph, etc… yours may be totally different depending upon mine and yours success with the lab. So don’t freak if your data is completely different from mine. And therefore the analysis of results sometimes may need to be answered differently since you base your analysis off your data and not mine. DISCLAMIER: DO NOT CHEAT! Introduction: The purpose of this lab experiment was to separate plant pigments using paper chromatography, and to measure the rate of photosynthesis in isolated chloroplasts. Because of capillary action the solvent moves up the paper causing the pigments to become visible at certain distances. The substances visible on the paper are called pigments. Chlorophyll a is the main pigment that makes up about 75% of the pigmentation in plants. Chlorophyll b makes up about 25% of the pigmentation. And carotenes and xanthophylls are accessory pigments that make up the rest of the pigmentation. Carotene is the most soluble of the pigments and as a result will be carried the farthest by the solvent. The paper will display a spectrum of the pigments found in the spinach leaves. Using the formula Rf one can determine the relationship between the distance the solvent traveled to the distance the pigment traveled. Rf=distance l2igment migrated (mm) distance solvent front migrated Light is a part of a continuum of radiation or energy waves. The energy from visible light is used in the photosynthetic process. Light is absorbed in the leaf pigments, electrons within each photosystem are boosted to a higher energy level to produce ATP and to reduce NADP and NADPH. The ATP is then used in carbon fixation.
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