Methods and Materials: In the pigments of photosynthesis lab two analytical tools were used: thin layer chromatography and a spectrophotometer to examine cyanobacteria, red algae, green algae, brown algae and light and dark barley. Thin layer chromatography will allow one to determine the environmental effects on pigment arrangement in the light and dark barley. A mortar and pestle were used to grind up the light-grown barley using about 4 leaves and 3 mL of methanol. The dark barely is treated the same, expect twice as many leaves were used. One must ensure to grind the leaves as much as possible so the pulp does not affect the spectrophotometer readings. Transfer the liquid from each type of barley into two separate 1.5 mL microcentrifuge tubes. Place the tubes in the centrifuge making sure they are on opposite sides of one another so the centrifuge is balanced. Turn on the centrifuge for 5 minutes. Locate the TLC plates and grab a TLC strip along with two capillary tubes. Place each capillary tube in
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