Chapter 12 - Introduc)on to spectroscopy: IR& ...

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Unformatted text preview: Introduc)on to spectroscopy: IR & Mass Spectrometry Electromagne)c radia)on Wavelength, frequency, wavenumber frequency wavelength Photon energy wavenumber Absorp)on spectroscopy Infrared spectroscopy IR absorp)on Factors that determine IR absorp)on Intensity Absorp)on posi)on Func)onal ­Group IR absorp)on Which is trans ­2 ­heptene and which is 2 ­methyl ­1 ­hexene? IR Spectrum of 1 ­hexanol Mass spectrometry: Of course proteins can fly! 2002 Nobel Prize in Chemistry "for their development of soL desorp)on ionisa)on methods for mass spectrometric analyses of biological macromolecules" John B. Fenn 1/4 of the prize USA Koichi Tanaka 1/4 of the prize Japan Electron ­impact mass spectrometer Fragmenta)on ion Fragmenta)on reac)on Isotopic peaks Fragmenta)on Chemical ­ioniza)on (CI) Mass spec instrument A FT ­ICR mass spectrometer Key Points •  Concepts of wavelength, wavenumber, frequency, and photo’s energy; •  Principles and conceptual instrumenta)on of absorp)on spectroscopy (e.g. IR); •  Bond vibra)on and characteris)c frequencies; •  Factors that determine IR absorp)on posi)on (mode of molecular mo)on, atom mass, bond strength) and intensity (dipole, concentra)on); •  IR absorp)ons for important func)onal groups; •  IR spectra analysis; •  Principles of mass spectrometry; m/z values; •  Fragmenta)on ion and fragmenta)on reac)on (explain spectra); •  Analysis of isotopic peaks; •  Chemical ionizaton ...
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