Lecture 2.26

Lecture 2.26 - Fig. 7-21 DNA Sigma factor Longer...

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Unformatted text preview: Fig. 7-21 DNA Sigma factor Longer transcripts Short transcripts Sigma RNA Promoter region Gene(s) to be transcribed (light green strand) RNA polymerase (core enzyme) Sigma recognizes promoter and initiation site Transcription begins; sigma released. RNA chain growth continues to termination site Termination site reached; chain growth stops Release of polymerase and RNA Fig. 7-22 Promoter sequence 35 sequence Pribnow box Consensus Sigma mRNA start Transcription RNA polymerase (core enzyme) 1. 2. 3. 4. 5. 6. Table 7-3 Fig. 7-23 DNA with inverted repeats Inverted repeat Transcription of lower strand Inverted repeat Fold to form secondary structure Stem-loop in RNA immediately upstream from a run of uracils leads to termination of transcription RNA Rho-dependent termination Rho factor ( et al ) binds to nascent RNA RNAP pauses at rho-dependent termination site on DNA template Rho complex mediates release of RNAP and RNA from template Mechanism found in Bacteria but not in Archaea Fig. 7-24Fig....
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Lecture 2.26 - Fig. 7-21 DNA Sigma factor Longer...

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