Lecture 3.15 Notes

Lecture 3.15 Notes - Notes Microbial Genomics Genomics of bacterial virulence Horizontal gene flow and genome evolution Metagenomics Advances in

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Notes – 3/15/10 Microbial Genomics Genomics of bacterial virulence Horizontal gene flow and genome evolution Metagenomics Advances in molecular technology o Shotgun cloning o Automated sequencing o Computational bioinformatics History Prokaryotic genomes: sizes and ORFs www.genomesonline.org priorities of pathogens and organisms useful for biotech industry size ranges o strong correlation between genome size and ORF content in prokaryotes – but not in eukaryotes where most of genome is in noncoding seqs o how many genes required for life? Smallest free-living genome (~1,400 genes) Smaller for parasites or endosymbiotic cells – smallest known Carsonella rudii @ 160 kbp Largest – Sorangium cellulosum @ 12.3Mbp Prokaryotic genomes: bioinformatics and gene distributions Sequencing annotation gene comparison with other organisms Complement of genes defines an organisms biology: o Treponema pallidum (syphilis) parasitic lifestyle reflected by lack of enzymes for amino acid biosynthesis in its genome o Termostable enzymes of hyperthermophiles Trends in gene comparisons o Metabolic genes most abundant ( in free-living organisms) o >30% hypothetical ORFs (w/ current bioinformatics tools) o changes in gene categories as a function of genome size Genomes of organelles Endosymbiotic origin of mitochondria and chloroplasts
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Lecture 3.15 Notes - Notes Microbial Genomics Genomics of bacterial virulence Horizontal gene flow and genome evolution Metagenomics Advances in

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