330-10 JP 15

# 330-10 JP 15 - BISC 330L Sp2010 Lect JP 15 Wednesday 17 Feb...

This preview shows pages 1–9. Sign up to view the full content.

BISC 330L Sp2010 Lect JP 15.ppt Wednesday, 17 Feb 2010 Petruska Lecture 14 (Enzyme Kinetics) Cont’d Lecture 15: Michaelis-Menten Equation, Linear Plots, and Reversible Inhibition Model Reference: BTS (6th ed.) Chap. 8

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
Biochemistry Sixth Edition Chapter 8: Enzymes: Basic Concepts and Kinetics Copyright © 2007 by W. H. Freeman and Company   Berg • Tymoczko • Stryer Sections 8.4-8.5: Enzyme Kinetics & Inhibition
Lock-and-key model of enzyme-substrate binding. (Binding energy holds substrate in pre-formed active site)

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
Induced-fit model of enzyme-substrate binding. (Some of the binding energy is needed to form the active site)
Determining initial velocity (i.e., when [P]<<[S]).

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
Curve of v 0 vs. [S] for Michaelis-Menten kinetics
Michaelis-Menten Model of Enzyme Kinetics Section 8.4 Equation (13), p.217 k 1 k 2 E + S ES E + P k -1 Assumptions: (a) k 2 represents catalytic rate (k cat ) for converting ES to EP, if catalysis is slow compared to P release. (b) k -2 can be ignored as long as [P] << [S],

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
Case 1: Binding withou t catalysis (k 2 = 0) ES quickly reaches equilibrium : d[ES] / dt = k 1 [E][S] - k -1 [ES] = 0 at equilibrium Then, k -1 [ES] = k 1 [E][S] (at equilibrium) i.e., k -1 / k 1 = [E][S] / [ES] = K D (or K d ) Eq. 1 where K D = k -1 / k 1 is the equilibrium dissociation constant for ES
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

### Page1 / 27

330-10 JP 15 - BISC 330L Sp2010 Lect JP 15 Wednesday 17 Feb...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document
Ask a homework question - tutors are online