This preview has intentionally blurred sections. Sign up to view the full version.
View Full DocumentThis preview has intentionally blurred sections. Sign up to view the full version.
View Full DocumentThis preview has intentionally blurred sections. Sign up to view the full version.
View Full Document
Unformatted text preview: BISC 330L Sp2010 Lect JP 16.ppt Friday, 19 Feb 2010 Petruska Lecture 15 (MM Equation, Linear Plots, and Reversible Inhibition Model) Cont’d Lect. 16: Classical Inhibitors & Catalytic Strategies Reference: BTS (6th ed.) Chap. 8, 9 Summary of Equations in MM Enzyme Kinetics he velocity equation, v = d[P]/dt, can be expressed as follows under steadystate conditions. a) v = k 2 [ES], the starting equation. At steadystate: [ES] = [E][S]/K M b) v = (k 2 /K M )[E][S], where (k 2 /K M ) indicates enzyme efficiency , when compared with k 1 . c) v = k 2 [E T ][S]/(K M +[S]) = V max [S]/(K M +[S]), = k 2 [E T ] = K D + ( k 2 / k 1 ) Curve of v vs. [S], a rectangular hyperbola predicted by MichelisMenten model. v = V max [S]/( K M + [S]) 1 8 Fig . 13.9 (LineweaverBurk Linear Eq., called “doublereciprocal” plot of MM Eq.) 8 4 1 {Note crowding of points 4 to 8) HanesWoolf Linear Eq. is more reliable. Fig. 13.10 1 4 8 (Note points 18 evenly spaced, in same order as original MM plot of v vs . [S].) If the MM model fits and V max is determined by catalysis (not by product release), then if enzyme is pure and [E T ] is known, k 2 = k cat = V max /[E T ] k cat , the turnover number, is the number of substrate molecules converted to produc per enzyme molecule per unit of time, when enzyme is saturated with substrate, i.e., when [ES] = [E T ]....
View
Full
Document
This note was uploaded on 08/30/2010 for the course BIO 320 taught by Professor Wu during the Spring '10 term at USC.
 Spring '10
 WU

Click to edit the document details