330-10 JP 17

330-10 JP 17 - BISC 330L Sp2010 Lect JP 17.ppt Monday, 22...

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BISC 330L Sp2010 Lect JP 17.ppt Monday, 22 Feb 2010 Petruska Lecture 16 (Reversible Inhibitors) Cont’d Reference: BTS (6th ed.) Chap. 8, 9,10
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General Model for Reversible Inhibition of Michaelis-Menten Kinetics
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If [E T ] and [I] are held constant, while [S] is varied , then v vs . [S] in the presence of [I] can be described by a Michaelis-Menten Equation of the general form, v = V I max [S] /(K I M + [S]) where V I max and K I M are constants related to original V max and K M as follows: V I max = V max / (1 + [I]/K I ’) K I M = K M (1 + [I]/K I ) / (1 + [I]/K I ’)
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Classical Types of Reversible Inhibitors Competitive (V max unchanged, K M increased) Noncompetitive (K m unchanged, V max decreased) Uncompetitive (V max and K m decreased by same fraction, so initial slope V max /K m unchanged)
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Classical Types of Inhibition (a) Competitive I binds to E but not to ES: K I ’ is infinite , so [I] / K I ’ is 0 for all values of [I], yielding V I max = V max (unchanged) K I M = K M (1 + [I]/K I ) (increased) Examples: Inhibitors binding in same place as S, e.g., analogs of substrate or transition state.
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330-10 JP 17 - BISC 330L Sp2010 Lect JP 17.ppt Monday, 22...

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