BISC 330 Spring 2010 Lecture 10

BISC 330 Spring 2010 Lecture 10 - BISC330L:Biochemistry...

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BISC 330L:  Biochemistry Spring Semester 2010 USC Lecture:  Wednesday, 03 February 2010 1. Finish Chapter 3:   Biochemistry Toolbox  Utilizing Antibodies  Synthesis of Peptides
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Compare to  peptide mass fingerprint   to find identity of protein Mass Spectrometry
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Structure of Antibodies
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Antibody v. Antigen Antibody =  immunoglobulin Antigen = foreign  substance Antigenic  Determinant =  epitope
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Types of  Antibodies Polyclonal Heterogeneous  mixtures of  antibodies Each antibody  specific for one  epitope Monoclonal Identical antibodies Specific for one  epitope
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The Western Blot
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The Southwestern Blot Renature proteins Incubate with 32 P labeled dsDNA probe Visualize  using X-ray film Identify  size of protein factor Using DNA to find protein
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The Indirect ELISA Used to detect presence of antibody
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INDIRECT ELISA:  E nzyme L inked I mmunosorbant A ssay Easy and fast method to detect and quantify antibody
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This note was uploaded on 08/30/2010 for the course BIO 320 taught by Professor Wu during the Spring '10 term at USC.

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BISC 330 Spring 2010 Lecture 10 - BISC330L:Biochemistry...

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