BISC 330 Spring 2010 Lecture 12

BISC 330 Spring 2010 Lecture 12 - BISC 330L: Biochemistry...

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BISC 330L: Biochemistry Spring Semester 2010 USC Lecture: Monday, 08 February 2010 1. Chapter 4: Flow of Genetic Information DNA Replication Transcription
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Fig. 4.16 Diagram of semi-conservative DNA replication.
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Density-Gradient Centrifugation: In terms of density, RNA > DNA > Protein > Lipid. A concentrated CsCl solution (density 1.7 gm/ml), containing cell extract, upon high-speed (utlra-) centrifugation (100,000 x g), creates a density gradient (1.6-1.8 gm/ml that gives very good separation of RNA and DNA from each other and from Proteins & Lipids. Lipids & Proteins float on top of the CsCl solution, while RNA and DNA form distinct bands in the solution, at the levels equal to their densities.
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14 N DNA and 15 N DNA in CsCl density gradient (Ultracentrifugation). To obtain 15 N DNA Meselson and Stahl grew E. coli for several generations in medium containing 15 NH 4 Cl as sole nitrogen source. Then they introduced 14 NH 4 Cl as nitrogen source. DNA
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This note was uploaded on 08/30/2010 for the course BIO 320 taught by Professor Wu during the Spring '10 term at USC.

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BISC 330 Spring 2010 Lecture 12 - BISC 330L: Biochemistry...

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