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Unformatted text preview: resides. 13. What is a heterotetramer? 14. Describe the quaternary structure of hemoglobin. 15. How does β-mercaptoethanol work? 16. Review the equations involved in predicting α and β structures. Use the mnemonic devices to remember the αand β promoting amino acids. 17. What are the various ways to generate a cell extract/homogenate? 18. Review the process of differential centrifugation. 19. Briefly discuss the various types of chromatography. Which method would you utilize to isolate an overall negatively charged protein? 20. In SDS-PAGE, why is protein separation based solely on size and not charge? 21. Draw a diagram of how isoelectric focusing is accomplished. Problems at the end of Chapter 3 (p. 104-105) #1, 10...
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- Spring '10
- negatively charged protein, pI calculation problems