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Study Questions for Week 3 - Class Version

Study Questions for Week 3 - Class Version - resides 13...

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STUDY QUESTIONS FOR WEEK 3: 1. Review pI calculation problems. 2. What is the difference between anti-parallel and parellel β sheets? 3. Briefly describe the hydrogen bonding that takes place in the two types of β sheets. 4. Briefly describe the hydrogen bonding involved in a β turn or hairpin turn. 5. Why are heptad repeats important for the formation of coiled-coil proteins? 6. Briefly describe the important structural features of the superhelical cable. 7. What is a motif? Give one example of a motif. 8. What is a domain? Give one example of a domain. 9. In class, we discussed a number of examples of tertiary structure. Discuss one of these examples. 10. What is an αβ barrel? 11. What is GFP? How can it be used to study proteins?
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12. Predict how amino acid distribution will be affected by the environment in which a protein
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Unformatted text preview: resides. 13. What is a heterotetramer? 14. Describe the quaternary structure of hemoglobin. 15. How does β-mercaptoethanol work? 16. Review the equations involved in predicting α and β structures. Use the mnemonic devices to remember the αand β promoting amino acids. 17. What are the various ways to generate a cell extract/homogenate? 18. Review the process of differential centrifugation. 19. Briefly discuss the various types of chromatography. Which method would you utilize to isolate an overall negatively charged protein? 20. In SDS-PAGE, why is protein separation based solely on size and not charge? 21. Draw a diagram of how isoelectric focusing is accomplished. Problems at the end of Chapter 3 (p. 104-105) #1, 10...
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Study Questions for Week 3 - Class Version - resides 13...

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