BIO%20311c%20Ch14-DNA-Structure%20%26%20Replication

BIO%20311c%20Ch14-DNA-Structure%20%26%20Replication - DNA...

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DNA Structure and Replication endelian enetics Mendelian Genetics Discovery of DNA as the Genetic Material DNA Structure Elucidation and Details NA Replication Process DNA Replication Process DNA Repair Mechanisms K. Sathasivan
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Basic Terms DNA Genes/ Locus Chromosome Genotype alleles G-A-T-C C-T-A-G AB, ab ,Ab mRNA rRNA/ tRNA Protein Gametes Diploid cells/tissue/ organism Phenotype Random fertilization Recombination, Segregation and independent assortment (brown eye, pink flower, Meiosis Mitosis sickle cell)
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Law of Segregation Allele pairs segregate during gamete formation and the paired condition is restored during fertilization.
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Figure 14.1 A genetic cross
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Mendel tracked heritable characters for three generations
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Mendel’s law of segregation
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Griffith’s Experiments
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Figure 11.2 Genetic Transformation by DNA (Part 1) Avery, McLeod and McCarty, 1944 – Transformation Experiment
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Figure 11.2 Genetic Transformation by DNA (Part 2)
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Hershey and Chase, 1952 – Phage Experiment
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Bacteriophage T2: Reproduction Cycle
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The Hershey–Chase Experiment (Part 1)
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The Hershey–Chase Experiment (Part 2)
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Figure 11.5 Transfection in Eukaryotic Cells
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Evidence so far riffith’s experiment ouse and R/S strain Griffith s experiment Mouse and R/S strain Avery and his group Transformation Hershey and Chase Phage with 32 P and 35 S NA can transform eukaryotic cells DNA can transform eukaryotic cells Charagaff’s rule % of A=T; % of G=C
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Elucidation of DNA Structure
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Nucleotides Have Three Components peatfig323here repeat fig 3.23 here
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5' end Deoxyribose (a 5-carbon sugar) Phosphate denine Adenine (A) Guanine (G) Purines (double-ring structures) Thymine ) (T) Pyrimidines ingle ng Cytosine (C) (single-ring structures) Fig. 14-4, p. 281 3' end
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DNA X-ray Diffraction
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Figure 11.7 Chargaff’s Rule
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This note was uploaded on 08/31/2010 for the course BIO 49810 taught by Professor Satasivan during the Spring '10 term at University of Texas.

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BIO%20311c%20Ch14-DNA-Structure%20%26%20Replication - DNA...

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