BioProj - Tracking Genes in Plants Anna Grace Martinson...

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Tracking Genes in Plants Anna Grace Martinson, Onuwa Nwosu, San Yuk Cheng, Esther Feng, Michelle Han, Hannah Velena, Obi Mbachu
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WHY? Research leads to better understanding genes of plants. This understanding can lead to identifying which genes control what phenotypes. Leads to other research.
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Seriously, WHY? To help feed the world! Increase the nutrients of plants. Make plants better in all aspects. Bend plants to our will.
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How? Well, we will walk you through a sample process. This process will isolate a particular gene to study in a plant. The isolated gene is injected back into the model plant and then studied to see how it affects the plant. Can study promoter region of DNA or target gene.
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First Step We need DNA from our organism! QuickTimeª and a decompressor are needed to see this picture.
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Isolation- Plasmid DNA from E.coli Grow E.coli cells that contain the plasmid you want to isolate. Use centrifuge to separate the parts. Alkaline lysis – name of the method Remove the pellet, then the supernatant to extract the DNA.
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Isolation-Plant Genomic DNA from Arabidopsis Tissue Size of nuclear DNA is very large Use micro centrifuge The method is to quickly freeze the tissue Maintain the ice-cold temperature Break the cell and cell wall QuickTimeª a decompress are needed to see
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Second Step Now we check our work. We want to be sure there were not mistakes made in the extraction process.
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Spectrophotometric Determination of DNA Quantity and Quality After isolation of DNA, quantification and analysis of quality are necessary to ascertain the approximate quantity of DNA obtained
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