BioProj - Tracking Genes in Plants Anna Grace Martinson...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Tracking Genes in Plants Anna Grace Martinson, Onuwa Nwosu, San Yuk Cheng, Esther Feng, Michelle Han, Hannah Velena, Obi Mbachu
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
WHY? Research leads to better understanding genes of plants. This understanding can lead to identifying which genes control what phenotypes. Leads to other research.
Background image of page 2
Seriously, WHY? To help feed the world! Increase the nutrients of plants. Make plants better in all aspects. Bend plants to our will.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
How? Well, we will walk you through a sample process. This process will isolate a particular gene to study in a plant. The isolated gene is injected back into the model plant and then studied to see how it affects the plant. Can study promoter region of DNA or target gene.
Background image of page 4
First Step We need DNA from our organism! QuickTimeª and a decompressor are needed to see this picture.
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Isolation- Plasmid DNA from E.coli Grow E.coli cells that contain the plasmid you want to isolate. Use centrifuge to separate the parts. Alkaline lysis – name of the method Remove the pellet, then the supernatant to extract the DNA.
Background image of page 6
Isolation-Plant Genomic DNA from Arabidopsis Tissue Size of nuclear DNA is very large Use micro centrifuge The method is to quickly freeze the tissue Maintain the ice-cold temperature Break the cell and cell wall QuickTimeª a decompress are needed to see
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Second Step Now we check our work. We want to be sure there were not mistakes made in the extraction process.
Background image of page 8
Spectrophotometric Determination of DNA Quantity and Quality After isolation of DNA, quantification and analysis of quality are necessary to ascertain the approximate quantity of DNA obtained and the suitability of DNA sample for further
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 08/31/2010 for the course BIO 49810 taught by Professor Satasivan during the Spring '10 term at University of Texas.

Page1 / 27

BioProj - Tracking Genes in Plants Anna Grace Martinson...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online