exam 3 gene - GENE 3200 Fall 2009 Bedell MWF Exam 3 Nov 12...

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GENE 3200 – Fall 2009 – Bedell MWF - Exam 3 – Nov 12 ID no. 810________________________ 1 Write your ID number (810 number, NOT SSN) on every page. Write your initials on the first page only. All of the multiple choice questions have only one correct answer. The exam should be completed in ink. Regrades will not be given if you write in pencil. All requests to re-grade an exam must be made in writing to Dr. Bedell within 48 hours of the grades being posted on WebCT. 1. (1 pt) True or false. The whole genome shotgun sequencing strategy does not require the generation of a minimum tiling path of BAC clones. 2. (1 pt) True or false. Paralogous genes arise by duplication within a species. 3. (2 pts) Which of the following statements IS true of fluorescent in situ hybridization (FISH)? (a) It is used for screening a genomic library. (b) It can be used to generate high-resolution physical maps of cloned genes. (c) It is used to localize cloned DNA fragments on karyotypes. (d) It requires the use of polymorphic DNA markers. (e) None of the above. 4. (2 pts) For genomic analyses, repeat sequences can be placed in five classes. Which of the following IS NOT one of these five classes? (a) Simple sequence repeats (b) segmental duplications (c) pseudogenes (d) transposon sequences (e) all of the above are repeat classes (f) none of the above are repeat classes 5. (2 pts) Which of the following statements DOES NOT describe high-density linkage maps? (a) They show the positions of genetic markers separated by less than 1 cM. (b) They are generated using data from analysis of SNPs and SSRs. (c) They show the order of cloned inserts of DNA. (d) They can be constructed using thousands of loci in a single pedigree. (e) All of the above describe high-density linkage maps. 6. (2 pts) Which of the following statements IS true of the hierarchical shotgun sequencing strategy to genome sequencing? (a) It requires generation of three genomic libraries cloned into plasmid and BAC vectors. (b) It requires generation of a minimum tiling path. (c) It requires generation of a cDNA library. (d) It was the strategy used by the private company Celera for generating a draft sequence of the human genome (e) All of the above 7. (2 pts) A bacterial auxotrophic mutant is ______________________________. (a) a strain that cannot grow unless auxin is provided in the media. (b) a strain that is unable to replicate autonomously. (c) a strain that cannot grow unless a specific nutrient is provided in the media. (d) a strain that cannot grow unless an antibiotic is provided in the media. (e) a strain that can grow only if an antibiotic is provided in the media. 8. (1 pt) True or false. The lysogenic cycle of phage production results in bacterial cell lysis. 9.
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This note was uploaded on 09/01/2010 for the course GENE 3200 taught by Professor Bedell during the Summer '10 term at University of West Georgia.

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exam 3 gene - GENE 3200 Fall 2009 Bedell MWF Exam 3 Nov 12...

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