quiz6 - GENE3200 Summer 2010 Bedell Quiz 6 July 23 ID no...

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GENE3200 – Summer 2010 – Bedell ID no. 810-________ KEY ______________ Quiz 6 – July 23 Bacterial cells of genotype pur pro + his + were transduced with phage P1 (a generalized transducing phage) grown on bacteria of genotype pur + pro his . Transductants containing pur + were selected and tested for the unselected markers pro and his. The numbers of pur + colonies with each of four genotypes are as follows: pro + his + 114 pro + his 160 pro his + 25 pro his 1 A. (2 pts) What is the co-transduction frequency (as percentage of total transductants) for pur and pro? Show your calculation. (25 + 1)/300 x 100 = 8.7% B. (2 pts) What is the co-transduction frequency (as percentage of total transductants) for pur and his? Show your calculation. (160+1)/300 x 100 = 53.7% C. What is the gene order? Justify your answer . pur – his – pro pur – pro – his pro – pur – his Co transduction results indicate that pur is closer to his than it is to pro. So, this rules out
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