test3 - GENE3200 Summer 2010 Bedell Exam 3 July 15 810 no._...

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GENE3200 – Summer 2010 – Bedell 810 no.______________________________ Exam 3 – July 15 1 Write your ID number (810 number, NOT SSN) on every page. Write your initials on the first page only. All of the multiple choice questions have only one correct answer. The exam should be completed in ink. Regrades will not be given if you write in pencil. All requests to re-grade an exam must be made in writing to Dr. Bedell within 48 hours of the grades being posted on WebCT 1. (2 pts) A plasmid used as a cloning vector in E. coli should have all of the following except : A. an ori sequence B. a selectable marker C. unique restriction sites D. inverted repeats E. all of the above should be present 2. (2 pts) In addition to restriction enzymes, which enzyme is required to insert a fragment of DNA into a cloning vector? A. DNA polymerase B. DNA ligase C. RNA polymerase D. reverse transcriptase E. topoisomerase 3. (2 pts) Which of the following statements is true of YACs? A. They are vectors used to clone DNA fragments up to 15 kb in Y. serbenius B. They are vectors used to clone DNA fragments between 250 and 2000 kb in Y. serbenius. C. They are vectors used to clone DNA fragments between 250 and 2000 kb in yeast. D. They are vectors used to clone DNA fragments between 10 and100 kb in yeast. 4. (2 pts) A DNA copy of an mRNA molecule is called a(n) ________. A. dDNA. B. cDNA. C. rDNA. D. mDNA. 5. (3 pts) Write the sequence for 6 nt primers that would amplify the entire DNA sequence shown below. Label each primer "forward" and "reverse". Don't forget the 5' and 3' labels! 5’-GTAATTGCCGGCTGGAATCGAATCGTAGGCATTAAAGCTAAGCTTGACCA-3’ 3’-CATTAACGGCCGACCTTAGCTTAGCATCCGTAATTTCGATTCGAACTGGT-5’ 6. (1 pt) True or false. In the dideoxy sequencing method, no further DNA synthesis takes place after insertion of ddNTPs into the new strand because the ddNTPs form a restriction enzyme site. 7. (2 pts) In the dideoxy (chain terminating) sequencing method, the reaction containing ddATP will terminate __________________________ A. opposite As in the template strand. B. opposite Ts in the template strand. C. opposite Gs in the template strand. D. opposite Cs in the template strand E. opposite any base selected randomly in the template strand.
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GENE3200 – Summer 2010 – Bedell 810 no.____________________________ Exam 3 – July 15 2 8. The figure below shows a plasmid vector (left) with unique sites for five restriction enzymes [XbaI (X), BamHI (B), EcoRI (E), HindIII (H), and SphI (S)]. In the plasmid the five restriction enzyme sites are located 0.1 kb apart in a multiple cloning site and the total size of the plasmid is 3.2 kb. To the right, is a genomic region of DNA the size (in kb) between each pair of restriction sites shown below the line. The position of a probe for Southern blots is shown above the genomic DNA fragment. 8 (cont) You want to clone the 14.8 kb HindIII fragment from this region of genomic DNA into
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This note was uploaded on 09/01/2010 for the course MIBO MIBO 3500 taught by Professor Dustman during the Summer '09 term at University of Georgia Athens.

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test3 - GENE3200 Summer 2010 Bedell Exam 3 July 15 810 no._...

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