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Lect12notes - Prokaryotic genetics The genetics of bacteria...

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Unformatted text preview: Prokaryotic genetics The genetics of bacteria and their viruses Ch.5 Molecular Biology 1. 2. 3 Normal roles 1. 2. 3 Prokaryotic systems : Reproduction:- asexual (cell division/growth)- clonal- variation? 1. 2. Recombination?- no meiosis - gene transfer 1. conjugation = 2. transformation = 3. transduction = !Transfer unidirectional! donor = recipient = partial diploid = The genetics of bacteria and their viruses Ch.5 Prokaryotic systems : Phenotypes/Selective systems prototrophic = auxotrophic = resistance sensitivity filter wash to remove penicillin auxotrophic (ade- ) prototrophic (ade + ) culture w/ adenine plate on minimal prototrophic (ade + ) auxotrophic (ade- ) culture in minimal plate on adenine + penicillin The genetics of bacteria and their viruses Ch.5 The genetics of bacteria and their viruses Ch.5 Crosses Donor (F + ) Recipient (F- ) pilus: F factor (plasmid) 5 3 3 Donor Recipient The genetics of bacteria and their viruses Ch.5 How? Conjugation Conjugation- Self transmissable plasmids: encode all functions- Mobilizable plasmids:-- - - smaller F plasmid 1. 2. 3. - 4. only transfer from F + to F- rapid (Why?) IS elements promote integration of F factor- insertion sequences = mobile DNA (~20)- - IS IS The genetics of bacteria and their viruses Ch.5 F plasmid...
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This note was uploaded on 09/01/2010 for the course BB 10294 taught by Professor Politz during the Spring '10 term at WPI.

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Lect12notes - Prokaryotic genetics The genetics of bacteria...

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