section III review(2)

section III review(2) - Locus control region Nucleosome...

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Chapter 18. Prokaryotic gene expression Steps of transcription; how to control the efficiency of RNA polymerase Prokaryotic genes are polycistronic: co-transcription (Operon) Operon; Promoter + Operator + Structural genes Lac operon: Lactose as an Inducer; Repressor (LacI product ; trans acting) Catabolite repression; master control for catabolism Concept of regulon Examples of operon -Lac operon; Ara operon (dual role of Ara C); Trp operon (stem-loop) ;Nif operon (Oxygen sensor) Alternative σ factors (examples of heat shock-induced genes) Utilization of marker genes (Marker gene fused with regulatory DNA (i.e.promoter) - addressing transcriptional regulation EMSA and protein labeling for DNA-protein binding assays
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Chapter 19. Eukaryotic gene expression Table 18.1: comparison between prokaryotes and eukaryotes Multiple RNA polymerase Promoter + Enhancers + (silencer) DNA binding motifs HLH activators-repressors (competition vs. qeunching) bHLH + Leucine Zipper (e.g. Myc/Max system)
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Unformatted text preview: Locus control region Nucleosome remodeling + DNA methylation epigenetics e.g. genomic imprinting Alternative splicing; miRNA Posttranslational regulation Sex determination as an examples Epigenetics Other than DNA sequence: Histones – methylDNA - RNA Histone code hypothesis certain “code” to direct gene activation/repression-encoder: Histone modifying enzymes-reader: modified Histone binding proteins -decoder: context of complexes RNA components -miRNA-siRNA-ncRNA methylDNA Crosstalk among players Experimental tools-bisulfite sequencing-chromatin immunoprecipitation-histone pull-down-epigenomics Chapter 20. Developmental genetics Classifications of mutations loss-of-function (null; hypomorphic; conditional; dominant-negative;RNAi) vs. gain-of-function (excessive/ectopic expression of genes) Experimental tools (localization of proteins) Extensive example – Drosophila Maternal effect Gap Pair-rule segment polarity / homeotic...
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This note was uploaded on 09/02/2010 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas.

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section III review(2) - Locus control region Nucleosome...

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