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Unformatted text preview: lacking threonine. 3. (2 points) Why did they choose that type of medium to plate on? 4. (2 points) In order for the above experiment to work, what must be true about the location of the thr gene? 5. (2 points) A cross is made between an Hfr strain that is met + arg + gly + and an F ‐ strain that is met ‐ arg ‐ gly ‐ . Interrupted ‐ mating experiments show that gly + enters the recipient last, so the gly + recombinants are selected on minimal plates supplemented with methionine and arginine. These recombinants are then tested for the presence of the met + and arg + alleles. The following numbers of colonies are found for each genotype: gly + met + arg + 420 gly + met + arg ‐ 11 gly + met ‐ arg+ gly + met ‐ arg ‐ 49 What is the gene order?...
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This note was uploaded on 09/02/2010 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.
- Spring '08