Homework-I - 3 Separate the RNA by size in an electrical...

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BIO 325 Genetics Homework 1 This homework 1 is due Monday, Mar. 2 (either after the class or by 5:00 pm to the BIO401 Drop box; no late homework accepted!); Coverage: Chapter 10-11 Please don't try to write on this sheet! Put your answers on another sheet of paper and turn that in. 1. (3 pts) What is meant by hierarchical shotgun sequencing strategy? 2. (1 pt) Below are several steps involved in FISH. From the choices available, pick the order of steps that would be successful. 1) Fix chromosomes and denature the DNA. 2) Arrest cells in metaphase. 3) Take a picture using ultraviolet light. 4) Drop cells onto microscope slide to open the cells. 5) Label a DNA probe bound to fluorescent dye. 6) Hybridize the DNA probe to the chromosomes. 4. (1 pt) Pick the order of steps involved in Northern blots that would be successful. 1) Blot the RNA from the gel to a filter. 2) Purify the RNA.
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Unformatted text preview: 3) Separate the RNA by size in an electrical field in an agarose gel. 4) Expose the filter to a labeled probe to allow hybridization. 5) Develop the film. 6) Perform autoradiography. 4-5) Allele specific oligonucleotides (ASO) can be used to determine the different alleles present in a DNA sample. Single sperm, which have completed meiosis, can be used for a polymerase chain reaction (PCR). The amplified DNA is then spotted to filters and hybridized with probes that recognize single nucleotide differences. Four primers (called A, B, D, and E) were used to amplify DNA samples and ASO probes were used to determine the allele present (either allele 1 or 2). "X" indicates the allele is present and "–" indicates the allele is not present. 4. (2 pt) Which loci are heterozygous? 5. (3 pts) What feature would distinguish an X-linked gene if you used PCR-amplified ASO markers in sperm?...
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This note was uploaded on 09/02/2010 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas.

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