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Unformatted text preview: 3) Separate the RNA by size in an electrical field in an agarose gel. 4) Expose the filter to a labeled probe to allow hybridization. 5) Develop the film. 6) Perform autoradiography. 4-5) Allele specific oligonucleotides (ASO) can be used to determine the different alleles present in a DNA sample. Single sperm, which have completed meiosis, can be used for a polymerase chain reaction (PCR). The amplified DNA is then spotted to filters and hybridized with probes that recognize single nucleotide differences. Four primers (called A, B, D, and E) were used to amplify DNA samples and ASO probes were used to determine the allele present (either allele 1 or 2). "X" indicates the allele is present and "" indicates the allele is not present. 4. (2 pt) Which loci are heterozygous? 5. (3 pts) What feature would distinguish an X-linked gene if you used PCR-amplified ASO markers in sperm?...
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- Spring '08