Exam 1-answers - Your Name Exam 1. BIO 325 Genetics Feb....

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Your Name Exam 1. BIO 325 Genetics – Feb. 16, 2009 1-8 ; Fill the blank with word(s) or numbers. (1 point ea.) 1. An origin of replication generally has _______ (how many?) replication forks. 2 2. The removal of an amino group from cytosine is known as ______________. Deamination 3. The usual flow of genetic information within a cell or organism is often called the "__________ ___________" of Molecular Biology. Central Dogma 4. There are _______ stop (nonsense; how many) codons recognized by ribosomes. The sequences of these codons are _______, ________, and ________. 3, UAA, UAG, UGA 5. Processing of eukaryotic RNA usually adds a(n) ___________ cap and a(n) _________ tail. 5’ methyl, poly(A) 6. AvaI (CPyCGPuG, Py stands for pyrimidine, Pu for purine) will cut DNA approximately every _________ bp. 1/4 X 1/2 X 1/4 X 1/4 X 1/2 X 1/4 = 1/1024; 1024 bp 7-10. You observed DNA replication in E.coli and drew diagram below. 7-8. Match the region or item on the diagram with the correct term 7. Lagging strand (1pt) C 8. DNA polymerase (1pt) b True or False; Circle one ( 1 pt ea.) 9. f from diagram is called Okazaki fragment T True False 10. Ligase is indicated in the diagram F True False 11. Pyrimidines are a nitrogenous base containing a double ring, while purines contain a single ring. (1pt) F True False
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Your Name 12. A region of DNA 100 bp in length has the potential to be represented by 4 100 unique sequences. (1pt) T True False 13. A cistron is any complementation group identified by the cis-trans test and is synonymous with a gene. T True False 14. cDNA libraries are generated using a reverse transcriptase and represent the population of RNA transcripts initially contained in the cells used. T True False 15. HIV does not contain DNA in its virus particles. T True False Multiple choice questions may have 1 ~ 5 correct answers. You should CIRCLE all correct answer(s) to be scored (no partial score); (2 pt ea.) 16. In the Hershey and Chase experiment designed to determine the molecule of heredity, what was radiolabeled with 35 S? A A. protein B. DNA C. RNA D. rRNA E. None of the choices are correct. 17. ________ bonds are responsible for the chemical affinity between A and T (or G and C) nucleotides. (1pt) C A. Ionic B. Covalent C. Hydrogen D. Electro-ionic E. None of the choices are correct. 18. _______ -form DNA spirals to the right and is the major form of naturally occurring DNA molecules. (1pt) B A. A B. B C. D D. Y E. Z 19. Replacing a thymine nucleotide with a guanine is an example of a C (C/D or C/E also considered right) A. translocation. B. transition. C. transversion. D. forward mutation. E. reversion or reverse mutation. 20. Assume that the mutation rate for a given gene is 5 × 10 -6 mutations per gene per generation. For that gene how many mutations would be expected if 10 million sperm are examined? D A. none B. 5 × 10 -6 C. 5 D. 50 E. 500 21. You set out to duplicate the Luria-Delbruck fluctuation experiment. You planted twenty small flasks with bacteria
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This note was uploaded on 09/02/2010 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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Exam 1-answers - Your Name Exam 1. BIO 325 Genetics Feb....

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