dna_intro_part_1b

dna_intro_part_1b - There are many enzymes that are...

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DNA Structure Primary genetic material is composed of two complementary strands Form a double helix or twisted ladder Sides are sugar phosphate and the steps are base pairs Four Bases- 2 Purines – Adenine and Guanine and 2 Pyrimidines- Cytosine and Thymine.
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DNA Structure Nucleotides are the building blocks themselves composed of PBS Nucleotides-PBS Phosphate (negative charge) Base (AGCT-Asian Guys Can Teach) Sugar (deoxyribose-5C) Phosphate-Sugars Connected by phosphodiester linkages
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DNA Structure 2 Complimentary, Antiparallel Strands held together by Base Pairs- H Bonds A:T held with 2 H Bonds G:C held with 3 H Bonds .
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Replication of DNA is Semi Conservative One old and one new http://dir.niehs.nih.gov/dirlmg/repl.html Enzymes of Replication DNA is replicated or copied in our cells. When completed, the new double strands consist of one old template and one newly made strand- This is called semi conservative replication.
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Unformatted text preview: There are many enzymes that are required. They include unwinding (helicases, gyrases), priming (primases), copying ( DNA polymerases) and touch up enzymes (DNA ligases). DNA replication is quite accurate in our cells. The error rate is approximately 10-9 O Base (A, T, C, or G) H O 1 3 2 4 5 CH 2 O P H H H H O O-HO O Base (A, T, C, or G) H OH 1 3 2 4 5 CH 2 O P H H H H O-HO P-P-OH dNTP 3-OH 5-P Template = Old Primer 5-P 3-OH DNA polymerase Mg++ PCR: repeated rounds of DNA Replication 5 required ingredients (components)- primer, template, Mg, dntps, DNA polymerase- PTMDD-(please to make DNA doubled) DNA Polymerase catalyzes the template directed (A-T, G-C), incorporation of dNTPs (PP is released) forming a 3-5 phosphodiester linkage Direction of synthesis 5 3 using primer 3OH to attach incoming nucleotide...
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dna_intro_part_1b - There are many enzymes that are...

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