Problem 1, 130C - deoxyribonucleotide, allows the frequency...

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Problem 1 (Due Wed., Feb. 17) Both DNA strands can be replicated in vitro using DNA polymerase I and dNTP mixtures in which one of the four deoxyribonucleoside triphosphates has its α -phosphate 32 P labeled. The product DNA can then be treated with Dnase I, an enzyme catalyzing the hydrolysis of phosphodiester bonds on the 3’ sides of all deoxyribonucleotides. Analysis of the mole fractions of the product 3’ labeled nucleotides from four sets of reactions, each using a different α - 32 P labeled
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Unformatted text preview: deoxyribonucleotide, allows the frequency of occurrence of each of the sixteen dinucleotides in the DNA (N 1 pN 2 ) to be determined. This technique (called nearest-neighbor analysis) was used to establish the antiparallel nature of the DNA duplex by determining which of the dinucleotides occurred at the same frequencies. Explain (using examples of the labeling patterns) how the results of this set of experiments can distinguish between the parallel and antiparallel models of DNA....
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This note was uploaded on 09/08/2010 for the course CHEM 130C at San Jose State University .

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