Folin-Ciocalteau

Folin-Ciocalteau - Chem 131A Folin-Ciocalteau Analysis for...

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Unformatted text preview: Chem 131A: Folin-Ciocalteau Analysis for Protein This method is quite sensitive. Samples containing as little as 5 ug of protein can be readily analyzed. The principle depends on the reaction of the phenolic (R) group of tyrosine with the colorless phosphomolybdotungstate ions in the presence of Cu 2+ ions. The phenol is oxidzed and the complex phosphomolybdotungstate is reduced to a blue chromogen that can be estimated spectrophotometrically. Important!! Steps 1 and 2 must be done at the same time. 1) Standard Tyrosine Curve: To obtain data for the curve, set up the following tubes: Tube 1 2 3 4 5 6 UNK UNK Standard tyrosine (mL) 0.10 0.20 0.30 0.40 0.50 0.30 0.50 Water (mL) 1.20 1.10 1.00 0.90 0.80 0.70 0.90 0.70 Absorbance Standard tyrosine solution: 100 μ g tyrosine/mL 2) Tyrosine Unknown. You will receive an unknown containing tyrosine plus an inert compound. The percentage of tyrosine varies You will receive an unknown containing tyrosine plus an inert compound....
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This note was uploaded on 09/08/2010 for the course CHEM 132L at San Jose State.

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Folin-Ciocalteau - Chem 131A Folin-Ciocalteau Analysis for...

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