Chem 130C, S09, Final Exam

Chem 130C, S09, Final Exam - Name ID # Chem 130C 1. a)...

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Name ID # Chem 130C Final Spring 2009 1. a) ( 10 points ) Determine the minimum number of macromolecules contained in a cell-free protein-synthesizing system derived from E coli, showing the reasoning that led to your answer. (Recall that the small and large subunits of prokaryotic ribosomes contain 21 and 31 component polypeptides, respectively.) b) ( 5 points ) Explain why prokaryotic ribosomes can translate a circularized mRNA molecule whereas eukaryotic ribosomes cannot, even in the presence of the required cofactors.
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Chem 130C Final Spring 2009 Page 2 2. a) ( 10 points ) Recall that the sense strand of the viral DNA (sequence shown below) encoded a five- residue peptide ( vir -2). In a mutant strain of the virus the T at position 23 of the sequence has been replaced with a G. Determine the amino acid sequence of the mutant version of vir -2. 5’-AGATCGGATGCTCAACTATATGTGATTAACAGAGCATGCGGCATAAACT-3’ b) ( 10 points ) Design an mRNA with the necessary prokaryotic control sites that encodes
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Chem 130C, S09, Final Exam - Name ID # Chem 130C 1. a)...

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